Anne Kneeland (Czechanski), Research Assistant III. I joined Dr. Reinholdt’s laboratory in Genetic Resource Science in January 2008 as her first hire! My position largely involves the derivation and characterization of embryonic stem cell lines from inbred and mutant strains and occasional gene targeting / CRISPR/cas9 editing of derived cell lines. Because strains vary in their response to culture, I work to expand and improve upon on our group’s culture and characterization protocols. I support various research projects that focus on molecular mechanisms of germ cell development, and in doing so regularly engage in immunohistochemistry, microscopy, flow cytometry, and molecular biology. Prior to joining the Jackson Laboratory, I worked in the laboratory of Dr. David Barnes at the Mount Desert Island Biological Laboratory where I focused on cell culture techniques in elasmobranchs and echinoderms. I received my B.A. from College of the Atlantic in 2006. While at College of the Atlantic I served as an INBRE fellow and completed my senior thesis in collaboration with Drs. James Denegre and Benjamin King at the Jackson laboratory, titled, “in drosophilo: Using Drosophila melanogaster as a tool to understand mouse oogenesis and early development”. I volunteer regularly at the local SPCA and have hiked 54 of New England’s 100 highest peaks.
Whitney Martin, Research Assistant II, I had the great fortune of joining the Jackson Laboratory in 2011 as an intern working for Dr. Kevin Seburn. I assisted in the research of Infantile Neuroaxonal Dystrophy (INAD), a rare but severe neurodegenerative disorder affecting infants 6-24 months old. Much of my time was spent extracting, quantifying and analyzing acetylcholine esterase activity at the neuromuscular junction (NMJ) of nm2913 mice as well as imaging the NMJ by confocal and electron microscopy. Upon the completion of my internship, I received my B.S. in Biology from Brigham Young University and accepted a position as a Research Assistant working in Genetic Resource Sciences for Dr. Cat Lutz. Under the direction of Laurent Bogdanik, I assisted in the generation and study of mouse models for a variety of neurodegenerative diseases, namely Amyotrophic Lateral Sclerosis, Duchenne’s muscular dystrophy, and Spinal Muscular Atrophy. I learned many skills such as DNA/RNA isolation, RT-PCR, Sequence analysis, genotyping, immunohistochemistry and colony management. Today I continue to work in the Genetic Resource Sciences under the direction of Dr. Laura Reinholdt. I am currently working on the derivation and characterization of embryonic stem cell lines from C57BL/6J<A/J>/NaJ consomic mice as well as the derivation of mouse embryonic fibroblasts from various strains of interest. In addition to cell culture, I’m also involved in the discovery of spontaneous mutations that cause Mendelian disease phenotypes in mice. I love to read and hike in my free time and I hope to learn at least 1 phrase in every language!
Narayanan Raghupathy, Ph.D., Computational Scientist (bio and pic coming soon)
Christopher Olivier, Research Assistant I (bio and pic coming soon)
Collaborating members of our team:
Belinda Harris, Phenotyping Specialist
Son Yong Karst, Senior Research Technologist
Louise Dionne, Senior Research Technologist
David Bergstrom, Ph.D., Research Scientist, Model Development